Tobacco Rattle Virus (TRV) Transmission by Sugar Beet Seeds
نویسندگان
چکیده
منابع مشابه
TRV–GFP: a modified Tobacco rattle virus vector for efficient and visualizable analysis of gene function
Virus-induced gene silencing (VIGS) is a useful tool for functional characterization of genes in plants. Unfortunately, the efficiency of infection by Tobacco rattle virus (TRV) is relatively low for some non-Solanaceae plants, which are economically important, such as rose (Rosa sp.). Here, to generate an easy traceable TRV vector, a green fluorescent protein (GFP) gene was tagged to the 3' te...
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Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing acetosyringone, cysteine, and Tween 20) that enables whole-plant level VIGS of (germinated) seeds in wheat and maize. Using the established system, phytoene...
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The rate of inactivation of tobacco rattle virus RNA by o.z M-nitrous acid at pH 4"o was comparable to that of tobacco mosaic virus RNA. In o'o025 M-nitrous acid at pH 6.0 this rate was greatly diminished, and was slower than that of intact virus. Possible reasons for this are discussed. Methods are described for the induction of mutants of tobacco rattle virus by nitrous acid, and for the sele...
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Beet necrotic yellow vein virus (BNYVV) and Beet soilborne mosaic virus (BSBMV) are members of the genus Benyvirus (9). Both viruses infect members of the family Chenopodiaceae, most notably sugar beet (Beta vulgaris L.), and are transmitted by the plasmodiophorid, Polymyxa betae (1,2). BNYVV infection causes rhizomania disease in sugar beet (4,21); however, BSBMV infection does not (28). BSBMV...
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Beet necrotic yellow vein virus (BNYVV) is a member of the genus Benyvirus (21,22) and causes the disease known as rhizomania. It often is reported to be the most important sugar beet (Beta vulgaris L.) virus (17). BNYVV is transmitted by the plasmodiophorid, Polymyxa betae Keskin (1,2,6). In the United States, the virus first was identified in California in 1984 (5) but now occurs in every maj...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2005
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2005.10817196